GENERATION OF PERINATAL-LIKE MESENCHYMAL STEM CELLS FROM HUMAN INDUCED PLURIPOTENT STEM CELLS

摘要

Non-naturally occurring perinatal mesenchymal stem cells differentiated from human induced pluripotent stem cells. The differentiation medium includes an amount of SB431542, an amount of BMP-4, and an amount of PD173074 and is repeated over the course of six days period until perinatal mesenchymal stem-like cells are formed. The resulting non-naturally occurring perinatal mesenchymal stem cells exhibit the relevant characteristics of naturally occurring perinatal mesenchymal stem cells, such as the positive expression of the mesenchymal stem cell markers CD73, CD90, and CD 105. The non-naturally occurring perinatal mesenchymal stem cells may be used to produce paracrine factors and similar compounds for therapeutic purposes.