METHOD OF MANUFACTURING A COLLAGEN-LAMININE MATRIX FOR THE HEALING OF SKIN ULASTERS, BURNS AND WOUNDS IN HUMANS

摘要

Process for the production of a collagen-laminin matrix for the healing of ulcers, burns and wounds of the human skin, including the production of a tissue-technical wound composition by combining techniques for the extraction of collagen from rat tail tendons, formation of collagen matrices, cultivation of immortalized keratinocytes of the skin of the HaCaT Line on a collagen substrate, characterized in that with the help of a solution of the detergents SDS (sodium dodecyl sulfate) and Triton-X-100 (octylphenyl polyoxyethylene) a decellurization (removal of cells from the end product) of the collagen film formed while preserving laminin (the main protein of the basement membrane ), which is synthesized by the cells of the HaCaT line, takes place on its surface.