Evaluation method, evaluation device and evaluation program for structural changes in proteins

摘要

PROBLEM TO BE SOLVED: To provide an evaluation method, an evaluation device and an evaluation program capable of easily evaluating a plurality of structural changes of a protein generated when an external stimulus is changed. SOLUTION: In the method for evaluating a structural change of a protein, an external stimulus to a protein sample is changed over time, and a fluorescence spectrum measurement and a CD spectrum measurement are performed at a plurality of timings during the change of the external stimulus (S3). , The procedure for calculating the two-dimensional correlation spectrum for each change over time of the measured fluorescence spectrum and the CD spectrum with the measurement wavelength of the fluorescence spectrum as the X-axis and the measurement wavelength of the CD spectrum as the Y-axis (S6), 2. A procedure for displaying the dimensional correlation spectrum as image data on the display means (S7), and a procedure for evaluating changes in the secondary structure and the tertiary structure of the protein sample based on the positive or negative peaks appearing in the two-dimensional correlation spectrum (S8). including. [Selection diagram] Fig. 4