EFFICIENT ANTIBODY DNA-BARCODING REAGENTS FOR MULTIPLEXED MOLECULAR IMAGING

摘要

Provided are DNA-barcoding reagents, DNA-barcoded antibodies, and methods of using DNA-barcoding reagents, and DNA-barcoded antibodies. The DNA-barcoding reagents comprise an affinity moiety that is specific towards antibodies, and covalently linked to one or more DNA sequences, optionally, by a linker. Also provided are methods of using the DNA-barcoded antibodies for, for example, multiplexed tissue antigen imaging and profiling, multiplexed biomolecule detection, and affinity purification and sorting of marker-positive cells.