CHROMATOGRAPHIC PURIFICATION OF AT LEAST ONE ENZYME SELECTED FROM THE GROUP CONSISTING OF COLLAGENASE TYPE I, COLLAGENASE TYPE II, NEUTRAL PROTEASE, AND CLOSTRIPAIN

摘要

The present invention relates to a method for purifying at least one enzyme selected from the group consisting of collagenase type I, collagenase type II, neutral protease and clostripain from a mixture of substances, said method comprising, as a method step, at least one hydrophobic interaction chromatography process, characterised in that the stationary phase in the hydrophobic interaction chromatography process comprises a material selected from the group consisting of polypropylene glycol and butyl-sepharose. The present invention also relates to the use of an enzyme purified in such a manner for pharmaceutical, cosmetic, and/or biochemical purposes.