SYSTEMS AND METHODS FOR COMPUTING THE CONTRIBUTIONS OF AUTOFLUORESCENCE IN MULTICHANNEL IMAGES

摘要

Disclosed herein are systems and methods of estimating the autofluorescence (AF) signal and other non-target signals in each channel of a multi-channel image of a biological sample that is stained with one or more fluorescent labels. In some embodiments, the estimated autofluorescence signal can then be subtracted or masked from the multi-channel image. In some embodiments, the autofluorescence-removed multichannel image can then be used for further processing (e.g. image analysis, etc.).