Method for improving the culture conditions of cultures of primary sweat gland cells and/or three-dimensional sweat gland equivalents

摘要

The present disclosure relates to a method for improving the culture conditions of cultures of primary sweat gland cells and/or three-dimensional sweat gland equivalents, where the culture with from about 500 to about 500,000 sweat gland cells and a diameter of from about 100 to about 6,000 nm is initially prepared and then cultivated in a culture medium with neurotransmitters and/or from about 11 to about 100 ng/ml of at least one growth factor. Cultivation of these cells and models in a culture medium containing at least one neurotransmitter and/or from about 11 to about 100 ng/ml of at least one growth factor leads to an increase of sweat-gland-specific marker proteins. As a result, a loss of function, which occurs with an absence of or only low expression of these markers, can be avoided.