GENETICALLY ENCODED FORMALDEHYDE REACTIVE UNNATURAL AMINO ACID, PREPARATION METHOD AND APPLICATION THEREOF

摘要

A genetically encoded formaldehyde reactive unnatural amino acid. Specifically, the present invention relates to a genetically encoded formaldehyde reactive unnatural amino acid PrAK, a preparation method and an application thereof in detection and imaging of formaldehyde. The genetically encoded formaldehyde reactive unnatural amino acid PrAK can specifically introduce sites into biomacromolecules such as a green fluorescent protein (EGFP) and firefly luciferase (fLuc) in the protein translation process, construct a reactive biomacromolecule formaldehyde fluorescent probe and a bioluminescent probe, and can effectively detect or image formaldehyde in a biospecimen.