METHOD FOR CAPILLARY ISOELECTRIC FOCUSING SEPARATION OF POLYPEPTIDES

摘要

In isoelectric focusing (IEF) separation, the resolution and focusing time is positively correlated to physical length of the separation channel. Thus, an increase in IEF resolution by means of increasing the length of the separation channel results in increase in separation time since each analyte must migrate a longer distance to be separated. The present disclosure provides a method for improved IEF resolution for a given amount of focusing time by using at least two isoelectric focusing sections of similar volume, but different diameters and linear lengths.