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Introduction of silencing activity into a dysfunctional RNA molecule and modification of specificity for a gene of interest
Introduction of silencing activity into a dysfunctional RNA molecule and modification of specificity for a gene of interest
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机译:将沉默活性引入功能障碍RNA分子和感兴趣基因的特异性修饰
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摘要
A method for producing an RNA molecule having silencing activity in a cell comprises: (a) RNA exhibiting a predetermined range of sequence homology that does not include complete identity to a nucleic acid sequence encoding the RNA molecule bound to RISC identifying a nucleic acid sequence encoding the molecule; (b) determining transcription of the nucleic acid sequence encoding the RNA molecule to select a transcribable nucleic acid sequence encoding the RNA molecule; (c) a small transcript of a transcribable nucleic acid sequence encoding an RNA molecule exhibiting a predetermined range of sequence homology to select a transcribable nucleic acid sequence encoding an aberrantly engineered RNA molecule exhibiting a predetermined range of sequence homology. (small) determining the processing capability (processability) into RNA; (d) altering the nucleic acid sequence of an aberrantly processed and transcribable nucleic acid sequence to bind to RISC and to confer processing capability on a small RNA complementary to a first target RNA or target RNA of interest step to make.
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