Production of dsRNA for pest protection in plant cells through gene silencing

摘要

A method is provided for producing in a plant cell a long dsRNA molecule capable of silencing a pest gene, said method comprising the steps of: (a) a plant nucleic acid sequence encoding a silencing molecule having a plant gene as a target selecting in the genome of a plant, wherein the silencing molecule is capable of recruiting RNA-dependent RNA polymerase (RdRp); and (b) altering the nucleic acid sequence of the plant gene to impart silencing specificity to the pest gene, wherein a transcript of the plant gene comprising the silencing specificity is capable of recruiting the RdRp. Forming base complementarity with a single molecule to produce the long dsRNA molecule capable of silencing the pest gene, thereby producing the long dsRNA molecule capable of silencing the pest gene, in the plant cell.