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CO-PRODUCTION METHOD FOR MULTIPROTEIN SYSTEM, CO-PRODUCTION SYSTEM FOR MULTIPROTEIN SYSTEM AND USE THEREOF

机译:多碳素系统的共制备方法,多素素系统的共生系统及其用途

摘要

Provided are a co-production method for a multiprotein system, a co-production system for a multiprotein system and use thereof. Specifically, a vector for expressing a bacteriolytic protein and a plurality of vectors for expressing proteins are co-transferred into a protein expression strain, so that the obtained co-expression strain expresses corresponding proteins and bacteriolytic protein. As growth of the co-expression strain, the generated bacteriolytic protein acts on the co-expression strain, so that the co-expression strain is subject to autonomous lysis, and the expressed proteins are released, thereby forming a multi-protein system which can be used for in vitro synthesis of proteins and/or compounds.
机译:提供了一种用于多粒蛋白系统的共制剂方法,用于多粒蛋白系统的共生产系统及其用途。 具体地,用于表达用于表达蛋白质的含有噬菌蛋白的载体和用于表达蛋白质的载体被共转移到蛋白质表达菌株中,使得所获得的共表达菌株表达相应的蛋白质和溶杆菌蛋白。 作为共表达菌株的生长,所产生的溶菌蛋白在共表达菌株上作用,使得共表达菌株受到自主裂解,并且表达的蛋白质被释放,从而形成多蛋白质系统 用于体外合成蛋白质和/或化合物。

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