REAGENT KIT AND METHOD FOR RAPID DETECTION OF DRUG RESISTANCE OF HELICOBACTER PYLORI

摘要

A reagent kit and a method for the rapid detection of the drug resistance of Helicobacter pylori: setting up different antibiotic concentration experimental groups and a negative control group, the different antibiotic concentration experimental groups referring to experimental groups in which different concentrations of antibiotics are added to a heavy water culture medium, and the negative control group referring to an experimental group without antibiotics; after incubation and centrifugation, washing and performing Raman detection on a sample; and, on the basis of the obtained Raman spectrum, calculating the C-D ratio as C-D/(C-D+C-H), the C-D of the different antibiotic concentration experimental groups minus the C-D ratio of the control group being the △C-D ratio, and comparing same with a critical value (-0.0075) to determine the sensitivity and drug resistance of Helicobacter pylori. A liquid culture method of incubating Helicobacter pylori is introduced, and a heavy water Raman technique is applied to the detection of Helicobacter pylori drug resistance, enabling more accurate and rapid determination of the sensitivity of Helicobacter pylori to antibiotics, and having great significance for guiding the medication and treatment of patients with Helicobacter pylori.