2 SARS-COV-2 RNA SYSTEM FOR IDENTIFYING SEVERE ACUTE RESPIRATORY SYNDROME CORONA VIRUS 2 SARS-COV-2 RIBONUCLEIC ACID RNA

摘要

A system for detecting the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a biological sample includes a sampling device, a dissolution chamber, a NASBA fluid network and an analytical instrument. The sampling device is configured to contain a sample comprising a pathogen target sequence for SARS-CoV-2. The dissolution chamber is configured to be in fluid communication with the sampling device for receiving a sample. The dissolution chamber is configured to dissolve the sample into a lysate. A NASBA fluid network is configured in fluid communication with the dissolution chamber to receive a lysate. The NASBA fluid network includes enzymes, forward primers and reverse primers for amplifying a predetermined gene sequence from a pathogen target sequence contained in the lysate. The forward primer has an oligonucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 13 and SEQ ID NO: 17. The reverse primer has an oligonucleotide sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 6, SEQ ID NO: 10, SEQ ID NO: 14 and SEQ ID NO: 18. The molecular beacon is configured to attach to a pathogen target sequence. The beacon has an oligonucleotide sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 7, SEQ ID NO: 11, SEQ ID NO: 15 and SEQ ID NO: 19 and a fluorophore. The assay instrument is configured to excite the molecular beacon when the molecular beacon is attached to the pathogen target sequence to signal the presence of the pathogen target sequence.