25-HYDROXYVITAMIN D ANALYSIS METHOD BASED ON IMMUNOPURIFICATION IN COMBINATION WITH MASS SPECTROMETRIC DETECTION

摘要

A 25-hydroxyvitamin D (25-(OH)D) analysis method based on immunopurification in combination with mass spectrometric detection, mainly comprising the following steps: preparing a biotin-labelled antibody; preparing a magnetic bead that is coated with the biotin-labelled antibody; enriching and purifying a sample; and performing mass spectrometric detection. In the analysis method, by using a high specificity of antibody affinity, 25-(OH)D can be specifically enriched and purified from complex biological samples, and the accurate detection of 25-(OH)D2 and 25-(OH)D3 is separately achieved; according to an antibody-coupled magnetic bead enrichment method in combination with mass spectrometric detection, the use efficiency of a liquid phase mass spectrometry system is greatly improved while high throughput, sensitivity, and accurate quantification are achieved.