New formyl transferase for post-translational modification of a protein and uses thereof

摘要

The present invention relates to a novel "protein amino-terminal formyl transfer enzyme" activity of "phage SPN3US" that infects and inhibits Salmonella spp. and Escherichia coli O157:H7, and a method for preparing and using the same. Also provided is a method for mass production and isolation of "protein amino terminal formyl group transfer enzyme" cloned into an expression recombinant vector and expressed in transformed cells. The novel "protein amino-terminal formyl-transferase" activity of the present invention is different from the existing fMet/N-degron for post-translational modification of the amino-terminal formylation. The formyl transfer enzyme according to the present invention may be usefully used to screen improved immunogenic and antigenic substances.