The present invention relates to a method for producing sophorolipid based on an oleic acid substrate using Candida bombicola LA-200 strain, and more specifically, sophorolipid, a natural surfactant, using Candida bombicola LA-200 strain (KCTC 13531BP). It relates to a method for producing sophorolipid based on an oleic acid substrate, which has the advantage of being able to mass-produce (sophorolipid), which can be used in cosmetics and pharmaceuticals, which are various industrial fields requiring surfactant activity.
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