De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Additionally, devices for the production of high quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized simultaneously using microfluidic assemblies. Additionally, the methods herein allow for the rapid construction of large libraries of long, high-quality genes. Devices for the preparation of large libraries of long, high-quality nucleic acids are also described herein.
展开▼