METHODS AND SYSTEMS FOR IN VIVO FULL-FIELD INTERFERENCE MICROSCOPY IMAGING

摘要

According to one aspect, the invention relates to a system (101) for in vivo, full-field interference microscopy imaging of a scattering three-dimensional sample. It comprises a full-field OCT imaging system (130) for providing en face images of the sample, wherein said full-field OCT system comprises an interference device (145) with an object arm (147) intended to receive the sample and a reference arm (146) comprising an optical lens (134) and a first reflection surface (133), and an acquisition device (138) configured to acquire a temporal succession of two-dimensional interferometric signals (I1, I2) resulting from interferences produced at each point of an imaging field; an OCT imaging system (110) for providing at the same times of acquisition of said two-dimensional interferometric signals, cross-sectional images of both the sample and a first reflection surface (133) of said full-field OCT imaging system (130); a processing unit (160) configured to determine a plurality of en face images (X-Y) of a plurality of slices of the sample, each en face image being determined from at least two two-dimensional interferometric signals (I1, I2) having a given phase shift; determine from the cross-sectional images provided by the OCT imaging system (110) at the times of acquisition of each of said two two-dimensional interferometric signals (I1, I2) a depth (z) for each en face image (X-Y) of said plurality of slices; determine a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths.