DETECTION OF DRUG-RESISTANT MYCOPLASMA GENITALIUM

摘要

Provided are methods, compositions and systems for detecting the presence or absence of nucleic acid targets, such as nucleic acids of macrolide-resistant Mycoplasma genitalium. In one embodiment, real-time Ct values determined for a wild-type sequence and for a drug resistance marker, each on an opposite strand of the same amplification product, are compared to determine the presence or absence of the drug resistance marker in nucleic acids of a test sample.