To provide high yield methods for producing 2-deoxyribose-5-phosphate from 2-deoxyribose and a phosphate donor in the presence of an acid phosphatase.SOLUTION: Disclosed herein is a method for producing 2-deoxyribose-5-phosphate from 2-deoxyribose and a phosphate donor in the presence of an acid phosphatase, where the orthophosphate concentration in the reaction solution at the start of the reaction is 8 mass% or less.SELECTED DRAWING: None
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