Device for measuring in vivo redox potential and method for measuring in vivo redox potential and method for verifying in vivo redox potential

摘要

In the culture cell, the concentration of the metabolite and the redox substance was measured, and the concentration in the cell could be measured by the fluorescence method, but it was difficult to measure the redox potential in vivo.For the equation to give the redox potential E by the equation of Nernst, the temperature is set to 37 ° C. (310 K), and the redox potential E of hydrogen / hydrogen ion is e = - 0.061 × Ph + 0.031 × It becomes pH 2 (V).For pH = 7.4, e = - 0.451 + 0.031 × It becomes pH 2 (V).The hydrogen gas index pH 2 of the subject is measured, and E is calculated using the above equation.According to the potential verification method of the present invention, it is possible to confirm the change of the potential associated with the change of the hydrogen gas index pH 2 by measuring the potential before and after blowing, and obtaining the linear regression straight line by blowing air containing air and hydrogen gas into the phosphate buffer. It is found that the calculated redox potential approximates the actual redox potential.Diagram