Human serum for cell culture medium for clinical growth of human adipose stromal cells

摘要

Problem to be solved: to provide a method for preparing a therapeutic composition.A process for providing adipose tissue samples obtained from human subjectsThe total survival rate is greater than or equal to 35% by B. acridine orange / propidium iodide or truncated blue pigment exclusion assayAnterior fat cellsFat derived mesenchymal stem cells with proliferative abilityMicrovascular endothelial cellsEndothelial progenitor cellsMonocyte andContains a mixture of vascular smooth muscle cells andContains no mature fat cellsAt least 1% of the nucleated cells in the mixture are separated from the adipose tissue sample to obtain defined separation products comprising interstitial angiofractions which are adipose derived mesenchymal stem cellsC. the process of combining the defined isolates with the proliferative medium to obtain stem cells grown to confluence for clinical growth of human adipose stromal cells for human clinical and therapeutic applicationsIncludingMethod.No selection