Method for Non-Invasive Monitoring of Fluorescent Tracking Agents with Background Separation Correction

摘要

A method of monitoring time-varying fluorescence emitted from a fluorescence agent from within a diffusely reflective medium having a time-varying optical property comprises at least two measurements obtained at one data acquisition time from the patient before and after administration of the fluorescence agent. The step of providing a measurement data set comprising a plurality of measurement entries comprising a detected Flr meas signal adjacent the diffusely reflective medium by a filtered photo detector during illumination of the diffusely reflective medium by the excitation-wavelength light and at least one selected from Contains one DR signal: , DR em , and DR em, the filtered signal. The method includes identifying a post-equilibrium portion of the measurement data set and converting each Flr meas signal of each measurement data entry within the post-equilibrium portion of the measurement data set in the diffusely reflective medium only emitted by the fluorescent agent. The method further comprises converting into an IF agent signal representing the detected fluorescence intensity. The disclosed method includes eliminating the effect of leakage of excitation-wavelength light and eliminating the effect of spontaneous fluorescence from the Flr meas signal.