COMPOSITION FOR CRYOPRESERVATION OF BOVINE REPRODUCTIVE CELLS AND CRYOPRESERVATION METHOD THEREFOR

摘要

Provided are a cryopreservation solution and a cryopreservation method for bovine germ cells such as bovine sperm. 0.3-0.9 w/w% of an amphoteric polymer electrolyte (floating polyamino acid) in which an appropriate amount of carboxyl groups are introduced by reacting succinic anhydride with respect to 50-99 mol% of the amino group of ε-poly-L-lysine with a number average molecular weight of 1,000 to 20,000 Use a cryopreservation solution in which 2-4 w/w% of glycerin and 2-4 w/w% of glycerin are dissolved in a physiological solution. A preferred embodiment of the cryopreservation method is to dilute bovine semen 2.5 to 10 times depending on the physiological solution, and to the suspension obtained by the primary dilution step maintained at 2 to 8° C. and the primary dilution step while maintaining at 2 to 8° C. It includes a secondary dilution step in which bovine semen is diluted 5 to 20 times by further dilution by dropwise addition of a physiological solution containing an amphoteric polyelectrolyte (an immobile polyamino acid) and glycerin.