A method for nuclear reprogramming using synthetic transcription factors

摘要

Problem to be solved: to provide a method for reprogramming mammalian somatic cells by adjusting the expression of endogenous cell genes. In addition, providing a kit for such use of cells obtained by such methods and for the use of such cells for the treatment of diseases suitable for stem cell therapy and for such use.(a) under sufficient conditions for reprogramming mammalian somatic cells into artificial pluripotent stem cellsAnd for a sufficient period of timeOr (b) under conditions sufficient to differentiate the somatic cell into a target cell of a cell type that is substantially different from the mammalian somatic cell populationAnd for a sufficient period of timeThe mammalian somatic cell population (starting cell) is brought into contact with the synthetic transcription factor.No selection