The present invention provides a methylglyoxal synthetase derived from Oceanithermus profundus DSM 14977 strain comprising a polypeptide strep-tag, a polynucleotide encoding the same, a recombinant vector comprising the polynucleotide, and the recombinant It relates to a transformed microorganism into which a vector is introduced and a method for producing methylglyoxal using the methylglyoxal synthetase.
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