PRE-FRACTIONATION FOR MASS SPECTROMETRIC ANALYSIS

摘要

The present invention relates to a method of fractionating a variety of analytes bound to a matrix, said method comprising: (a) eluting said analytes with a plurality of eluents, wherein a first elution step is effected with an elution buffer comprising a first numbered eluent, and a subsequent (n-th) elution step is effected with an elution buffer comprising an n-th numbered eluent, wherein said n-th numbered eluent has a lower dipole moment and/or a higher hydrophobicity than said first numbered eluent.