METHOD FOR EFFICIENTLY SEPARATING AND PURIFYING RECOMBINANT HUMAN COAGULATE FACTOR VIII FC FUSION PROTEIN

摘要

Disclosed is a method for efficiently separating and purifying recombinant human coagulate factor VIII Fc fusion protein. The method comprises steps of affinity chromatography and anion exchange chromatography; and the sample captured by means of the affinity chromatography is eluted with a salt ion buffer containing 5％-20％ polyol organic solvents under the condition of pH 4.0 to 8.0, and the protein sample can be separated and purified to 85％ or more by further ProteinA affinity chromatography. The purification method is simple to operate, naturally connects each step of chromatography, has a high recovery rate and low cost, and easily increases production.