首页> 外国专利> METHOD FOR PRODUCING CELL-CONTAINING EXTRACELLULAR MATRIX, CELL CULTURE METHOD, DEVICE FOR PRODUCING CELL-CONTAINING EXTRACELLULAR MATRIX AND CONTROL PROGRAM

METHOD FOR PRODUCING CELL-CONTAINING EXTRACELLULAR MATRIX, CELL CULTURE METHOD, DEVICE FOR PRODUCING CELL-CONTAINING EXTRACELLULAR MATRIX AND CONTROL PROGRAM

机译:生产含细胞的细胞外基质,细胞培养方法,用于生产含细胞的细胞外基质和控制程序的装置的方法

摘要

A method for producing a cell-containing extracellular matrix, said method comprising: preparing an extracellular matrix solution, which contains (i) an extracellular matrix precursor and (ii) cells or a cell mass, inside a pipette having a tip opening; discharging the extracellular matrix solution; forming a drop of the extracellular matrix solution at the tip opening of the pipette; bringing the tip opening of the pipette close to a culture surface of a cell culture container; placing the drop on the culture surface while avoiding contact between the tip opening of the pipette and the culture surface; moving the tip opening of the pipette away from the culture surface; separating the drop from the tip opening of the pipette; and gelling the extracellular matrix solution to thereby form a cell-containing extracellular matrix.
机译:一种制备含细胞的细胞外基质的方法,所述方法包括:制备细胞外基质溶液,其含有(i)细胞外基质前体和(II)细胞或细胞质量,在具有尖端开口的移液管内; 放电细胞外基质溶液; 在移液管的尖端开口处形成细胞外基质溶液; 将移液管的尖端打开接近细胞培养容器的培养表面; 将下降放在培养物表面上,同时避免移液管的尖端开口和培养表面之间的接触; 将移液管的尖端打开远离培养表面; 将下降从移液管的尖端开口分开; 并胶凝细胞外基质溶液,从而形成含细胞的细胞外基质。

著录项

  • 公开/公告号WO2021201243A1

    专利类型

  • 公开/公告日2021-10-07

    原文格式PDF

  • 申请/专利权人 JSR CORPORATION;

    申请/专利号WO2021JP14208

  • 发明设计人 SUEMASA DAICHI;HAYASHI EIJI;

    申请日2021-04-01

  • 分类号C12M3;C12N5/02;

  • 国家 JP

  • 入库时间 2022-08-24 21:33:28

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