Positive control for analysis of pospiviroidae and its use

摘要

PROBLEM TO BE SOLVED: To provide a non-infectious positive control of pospiviroidae. Nucleic acid constructs are for real-time RT-PCR, are non-infectious to the host, can be amplified with a PCR primer set for cDNA amplification of pospi viroids, and regions to be amplified are pospi. It has a base sequence different from that of viroid, and the difference in the length of the base sequence is within the range of ± 5%, and the difference in the GC content of the base sequence is within the range of ± 5%. [Selection diagram] None