The present disclosure provides a primer and test kit used for SARS-CoV-2 gene amplification, and in combination with gene amplification systems comprising LAMP or RT-LAMP and the like, can identify COVID-19 patients and asymptomatic carriers, and determine whether the SARS-CoV-2 virus is present in environmental samples, etc.; complicated instruments are unnecessary, and optionally, the test results can be read with the naked eye; thus the invention is suitable for large-scale SARS-CoV-2 virus screening. The present disclosure also provides a method and application of said primer and test kit.
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