Humanization method based on three-dimensional structure

摘要

PROBLEM TO BE SOLVED: To provide a method for humanization of a non-human antibody using a structure-based scoring matrix. A method comprising the following steps: (a) aligning the amino acid sequence of a non-human heavy chain or light chain variable domain with a first humanized variant of the non-human heavy chain or light chain variable domain, ( b) An aligned framework position in which the non-human heavy or light chain variable domain and the first humanized variant of the non-human heavy or light chain variable domain have different amino acid residues, and because of the difference. , The step of identifying the position affecting the antigen binding and / or three-dimensional structure of the variable domain; (c) the non-human heavy chain or light chain variable by substituting the amino acid residue identified in step (b). The step of modifying the first humanized variant of the domain; (d) the step of producing a nucleic acid sequence encoding the modified variable domain, thereby producing a nucleic acid sequence encoding the humanized immunoglobulin variable domain. [Selection diagram] None