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Norovirus lateral flow analyzer using time-resolved fluorescence analysis and measurement method using this

机译:诺罗病毒横向流动分析仪使用时间分辨荧光分析和测量方法使用此方法

摘要

PROBLEM TO BE SOLVED: To provide a norovirus lateral flow analyzer and a method using time-resolved fluorescence analysis which has high sensitivity to norovirus GI and GII and can be rapidly detected in the field. SOLUTION: This is a norovirus lateral flow analyzer that detects and measures at least two or more kinds of norovirus gene groups at the same time independently, and comprises a first norovirus gene group analysis module 10 for detecting a first norovirus gene group. The second norovirus gene group analysis module 100 for detecting the second norovirus gene group is included, and the first norovirus gene group analysis module and the second norovirus gene group analysis module are on different porous membranes 18 and 118, respectively. Fluorescent signals are measured with a time delay after light irradiation on the first and second solid phase detection lines 21 and 121 to which a fluorescent label having a fluorescence emission lifetime longer than about 1 μs is bound, and at least two or more kinds are measured. Norovirus genes can be detected and measured independently at the same time. [Selection diagram] FIG. 3a
机译:要解决的问题:提供一种使用时间分辨荧光分析的诺罗维病毒横向流动分析仪,其对诺罗病毒GI和GII具有高敏感性,并且可以在现场迅速检测到。解决方案:这是一种诺罗病毒横向流分析仪,其在独立地检测和测量至少两种或更多种诺病毒基因组,并且包括用于检测第一诺病毒基因组的第一诺病毒基因组分析模块10。包括用于检测第二NOROVIRUS基因组的第二诺诺病毒基因组分析模块100,并且第一诺病毒基因组分析模块和第二炔病毒基因组分析模块分别在不同的多孔膜18和118上。在第一和第二固相检测线21和第二固相检测线21和121上的光照射之后用时间延迟测量荧光信号,其中荧光发射寿命长于约1μs的荧光标记被束缚,并且测量至少两种或更多种。可以同时检测和测量诺罗病毒基因。 [选择图]图。 3A

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