HELICOBACTER PYLORI FERRITIN-BASED NOVEL CORONAVIRUS S PROTEIN SINGLE-REGION SUBUNIT NANOVACCINE

摘要

Provided is a Helicobacter pylori ferritin-based novel coronavirus S protein single-region subunit nanovaccine. According to the present invention, a receptor binding domain (RBD) of a virus is used as an antigen and is connected with a Helicobacter pylori polymeric protein (HP_Ferritin) to form a fusion protein RBD-FP-HP_Ferritin, such that antigen multimerization is realized; and a eukaryotic cell expression system is then utilized for expression, so as to form a 24-mer nano-antigen by means of the self-assembly action of the HP_Ferritin. According to the solution, the defect that RBD monomers are insufficient in immunogenicity can be overcome; the obtained vaccine can remarkably improve the level of neutralizing antibodies of a host to viruses; and the generated antibodies have the capacity to strongly prevent viruses from invading target cells. Moreover, the vaccine provided by the present invention is simple in preparation method, easy to purify and high in safety, and can be quickly applied to clinical trials.