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PREPARATION METHOD FOR AND APPLICATION OF GENETICALLY ENGINEERED ANTITUMOR MICROPARTICLE
PREPARATION METHOD FOR AND APPLICATION OF GENETICALLY ENGINEERED ANTITUMOR MICROPARTICLE
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机译:基因工程抗肿瘤微粒的制备方法和应用
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摘要
The present invention provides a preparation method for a genetically engineered antitumor microparticle. The method comprises the following steps: S1, constructing recombinant plasmids of an over-expressed GM-CSF and a trans-membrane over-expressed GM-CSF; S2, obtaining ex-vivo tumor cells to cultivate primary tumor cells, and obtaining the primary tumor cells; S3, packaging lentiviral particles of the over-expressed GM-CSF and the trans-membrane over-expressed GM-CSF; S4, infecting the primary tumor cells, obtained in step S2, with the lentiviral particles to obtain primary tumor cells of the over-expressed GM-CSF and the trans-membrane over-expressed GM-CSF; S5, irradiating the primary tumor cells obtained in step S4 and a culture solution using an X-ray, and collecting a supernatant liquid after radiotherapy to obtain a mixture of a required microparticle and apoptotic tumor cell fragments; and S6, performing centrifugation on the mixture obtained in step S5. The present invention can solve the technical problems of poor effect, many side effects, proneness to drug resistance, and inhibition of organism immunity in conventional local chemotherapy.
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