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Methods and Compositions for Detecting Virulent and Avirulent Escherichia coli Strains
Methods and Compositions for Detecting Virulent and Avirulent Escherichia coli Strains
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机译:检测毒力和无毒大肠杆菌菌株的方法和组合物
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摘要
Disclosed herein are methods for detecting virulent Shiga toxin-producing E. coli (STEC) strains O26, O103, O121, and O111 in a biological sample comprising the steps of: (i) enriching the bacterial concentration of the biological sample to result in an enriched sample; (ii) isolating DNA from said enriched biological sample; and (iii) detecting virulent strain in said isolated DNA sample via real-time PCR and a melt curve assay. Also disclosed are primers for said assay, as well as kits comprising said primers.
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