SYSTEMS AND METHODS FOR SPECTRAL IMAGING CHARACTERIZATION OF MACROPHAGES FOR USE IN PERSONALIZATION OF TARGETED THERAPIES TO PREVENT FIBROSIS DEVELOPMENT IN PATIENTS WITH CHRONIC LIVER DISEASE

摘要

The present invention includes a method of macrophage phenotype profiling for the assessment, determination, and stratification of risk of development of fibrosis and/or cirrhosis within the liver, and treatment thereof, comprising the steps of: (a) obtaining a liver biopsy sample from a subject; (b) using fluorescently labeled antibodies to analyze the sample, by laboratory assay, for marker identification and expression comparison of one or more macrophage profiling markers relative to the level of expression of a macrophage profiling marker in at least one control or standard sample, and (c) using spectral analysis to correlate the fluorescent signal generated by the macrophage profiling marker/antibody complex to the risk of developing fibrosis and/or cirrhosis, and treating with anti-viral agents or changes in diet, weight loss, or reduction of fat consumption.