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METHOD OF DETECTING AN ENDOTOXIN USING LIMULUS AMEBOCYTE LYSATE SUBSTANTIALLY FREE OF COAGULOGEN
METHOD OF DETECTING AN ENDOTOXIN USING LIMULUS AMEBOCYTE LYSATE SUBSTANTIALLY FREE OF COAGULOGEN
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机译:使用基本上不含凝固性的氧琥珀细胞裂解物检测内毒素的方法
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摘要
The present invention is related to a method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising: (a) contacting the sample with a reagent comprising limulus amebocyte lysate (LAL) and a chromogenic substrate; and (b) measuring a chromogenic effect resulting from a change in the chromogenic substrate in the presence of endotoxin in the sample; wherein the LAL is substantially free of coagulogen. The method also relates to compositions and kits comprising LAL substantially free of coagulogen, and methods of making such.
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