Detection of carbapenemase- producing enterobacteriales, pseudomonas and acinetobacter species using a chromogen

摘要

A method for detecting the presence of carbapenemase-producing Enterobacteriales, Pseudomonas and Ancinetobacter in a sample, comprising; providing a sample suspected of having carbapenemase-producing Enterobacteriales, Pseudomonas or Acinetobacter, reacting the sample with a solution of the chromogenic compound HMRZ-98 ((7R)-7-[(z)-2-(2-aminothiazol-4-y1)-(Z)- 2(methoxyimino)acetamidel-3-(2,4-dinitrostyry1)-3-cephem-4 carboxylic acid)) or a salt thereof, and detecting a colour change (see figures 1-3) in the reaction medium when carbapenemase-producing Enterobacteriales, Pseudomonas or Acinetobacter are present in the test sample. The colour change may be yellow to red. The sample may be biological e.g. urine. The sample may be lysed prior to or simultaneously with reaction with the compound or salt thereof. Lysis may be facilitated by a polymyxin, glycopeptide or polypeptide antibiotic. The method may also use ESBL inhibitors (e.g. clavulanic acid), AmpC inhibitors (e.g. cloxacillin), carbapenems or penems. The compound or salt thereof can be dissolved in a solution with an organic solvent (e.g. DMSO), an organic acid (e.g. a linear saturated dicarboxylic acid) or zinc sulphate. A kit is disclosed with a microtitre plate with wells coated in the chromogenic compound or salt thereof or an assay disc impregnated with the chromogenic compound or salt thereof, which is provided with an ESBL inhibitor and/or AmpC inhibitor.