Method for identifying enterococci in sea water and marine sediments

摘要

The present invention relates to the field of marine environment monitoring, and more particularly to a method for identifying enterococci in sea water and sediments. A method for identifying enterococci in seawater and sediments, comprising the steps of: (1) taking liquid seawater samples and solid marine sediment samples, respectively, in the marine environment; (2) mixing the solid marine sediment samples with the physiological saline according to a certain ratio, letting them pass the mixture through the filter membrane; (3) One side of the filter membrane collecting the bacteria is directed upwards, and the other side is placed on the Slanetz & Bartley culture substrate for cultivation; (4) Transfer the filter membrane using a pair of tweezers to a plate with pre-warmed enterococcal agar culture substrate, and for the plate color development is carried out; (5) Selecting color colonies and using a hydrogen peroxide solution for a catalase experiment, and the colonies with negative catalase experiment are the enterococci. The method according to the present invention has good accuracy and low cost for the screening of enterococci and can be useful for various kinds of environmental samples of sea water and marine sediments.