In one aspect, the present disclosure provides a composition for reactivating freeze-dried probiotics to impart a negative zeta potential to the probiotic cell surface, and L-lysine, L-ornithine, L-tyrosine and L-histidine as reactivators It relates to a Zeta-bio composition comprising at least one selected from the group consisting of. By activating freeze-dried probiotics, the present disclosure exhibits negatively charged zeta potential, improves intestinal viability and intestinal adhesion of freeze-dried probiotics, and has the effect of recovering cell damage caused by freeze-drying.
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