首页>
外国专利>
Method for highly efficient conversion of human stem cells to lineage-specific neurons
Method for highly efficient conversion of human stem cells to lineage-specific neurons
展开▼
机译:用于高效转化人干细胞对谱系特异性神经元的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to the field of stem cells. More specifically, the present invention provides methods and compositions useful for the highly efficient conversion of human stem cells to lineage-specific neurons. In a specific embodiment, a method of inducing differentiation of human stem cells into dopaminergic (DA) neurons comprises the steps of (a) transfecting human stem cells with a lentiviral vector encoding Atoh1, wherein the vector is Dox inducible; and (b) growing the transfected cells in culture in the presence of Dox, Sonic Hedgehog (SHH) and FGF-8b until DA neurons are induced.
展开▼