Measuring method of brain natriuretic peptide and measurement kit of brain natriuretic peptide

摘要

The present invention relates to a method of measuring a BNP capable of measuring a concentration of BNP in an analyte with higher sensitivity and higher reproducibility than in a conventional measuring method.A pretreatment sample is obtained by adding protease inhibitor to the sample containing the brain natriuretic peptide.Said pretreatment sample is provided on a metal membrane immobilized with a binding material that specifically binds to a brain natriuretic peptide to bind said brain natriuretic peptide contained in said pretreatment sample.The brain natriuretic peptide is labeled with a fluorescent substance before or after binding to the binding substance.In the state where the brain natriuretic peptide labeled with the fluorescent substance is coupled to a binder material fixed to the metal film, excitation light is applied to the metal film so that surface plasmon resonance occurs in the metal film, and fluorescence emitted from the fluorescent substance is detected.