CELL CULTURE SUBSTRATE, METHOD FOR PRODUCING CELL CULTURE SUBSTRATE, AND METHOD FOR PRODUCING SPHEROIDS

摘要

There are provided a cell culture substrate that enables efficient spheroid formation for cells and can form spheroids having a uniform size and an arbitrary shape at a high cell viability, a method for producing the cell culture substrate, and a method of producing spheroids in which the cell culture substrate is used and a cell viability inside of the spheroids is excellent. A cell culture substrate includes a substrate and a stimulus-responsive polymer coated on the substrate, wherein the stimulus-responsive polymer is a block copolymer having a water-insoluble block segment and a stimulus-responsive block segment, and the cell culture substrate includes the following two regions (A) and (B): (A) an island-shaped region having cell proliferation properties and stimulus responsiveness and having an area of 0.001 to 5 mm²; and (B) a region adjacent to the region (A) and having no cell proliferation properties.