PROCEDURES FOR REGULATING IN-VITRO BIOSYNTHESIS ACTIVITIES BY NUCLEAR SYSTEM KNOCKOUT

摘要

Provided is a method for regulatingin vitrobiosynthesis activity by knocking-out of a nuclease system, comprising: screening five nucleases among several nucleases, and performing down-regulation or knocking-out on one of the five nucleases (such as EXN53). The method can improve the stability of nucleic acid and the protein production efficiency of anin vitroprotein synthesis system.