METHOD AND DEVICE FOR HIGH-RESOLUTION FLUORESCENCE MICROSCOPY

摘要

The invention relate to a method for high-resolution fluorescence microscopy, wherein a number N of individual images of a sample (1), marked by fluorophore and excited to fluorescence, is captured, wherein the sample (1) is illuminated by N different effective lighting patterns in succession, and the individual images are combined into an composite image having higher structural resolution than in the individual images, and the composite image is then output, wherein each effective lighting pattern is generated from the superimposition of at least two basic lighting patterns, wherein the basic lighting patterns each differ from each other and for each individual image, and wherein the fluorophors are excited to fluorescence in the sample (1) only where the at least two basic lighting patterns overlap, illuminating the sample (1), wherein the illuminating superimposition of the at least two basic lighting patterns triggers non-linear excitation and/or emission effects and/or switching effects in the flurorophors.