SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2

摘要

FIELD: medicine.;SUBSTANCE: invention relates to the field of medicine and molecular biology, namely to the molecular genetic diagnosis of COVID-19 based on the detection of RNA of the SARS-CoV-2 coronavirus. The synthesized oligonucleotide primers and fluorescent probes are complementary exclusively to the nucleotide sequence (ORF1ab and N region) of the genome of the SARS-CoV-2 coronavirus and the human ABL1 gene. Also developed a method for detecting SARS-CoV-2 coronavirus RNA using multiplex RT-PCR based on amplification of ORF1ab and N regions of the SARS-CoV-2 coronavirus gene and human RNA ABL1 gene as an internal control.;EFFECT: invention allows reliably confirming the clinical diagnosis of COVID-19 with a minimum amount of the test material, 10 mcl, by monitoring the quality of sampling and RNA isolation by amplifying the RNA of the human ABL1 gene and determining the presence/absence of SARS-CoV-2 coronavirus RNA by amplification relative to control samples.;2 cl, 5 dwg, 6 tbl, 5 ex