INTEGRATION VECTOR FOR MULTI-COPY GENE INTEGRATION IN 18SPPHK OF PICHIA PASTORIS YEAST

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the field of biotechnology and genetic engineering. Proposed is an integration vector pPIG-1 for expression of recombinant proteins in the P. pastoris yeast, with a size of 5595 bp, characterised by a nucleotide sequence disclosed in SEQ ID NO 1.;EFFECT: invention provides a high frequency of target gene integration. ;1 cl, 2 dwg, 3 ex, 2 tbl