Culturing Method for cultured root of Leguminous plants comprising high-content of coumestrol

摘要

The present specification relates to a method for culturing legume cultured roots having an increased content of coumestrol capable of producing a large amount of coumestrol present in a very trace amount in legumes, the culture method comprising: (a) culturing a legume seed Inducing an in-flight plant in which cotyledons, hypocotyls and rhizomes are produced by germinating in a medium; (b) culturing any one or more sites among cotyledons, hypocotyls, and milk roots of the induced in-flight plants in a culture medium to induce cultured roots for each site; and (c) culturing the induced cultured roots for each site in a culture medium, and adjusting the air supply amount and air composition to proliferate; Including, the culture medium is NH ₄ NO ₃ , CaCl ₂ · 2H ₂ O, MgSO ₄ · 7H ₂ O, KH ₂ PO ₄And KNO _{3 It} relates to a medium comprising a nutrient component, to a method.